Trends in Clinical and Medical Sciences
Special issue: Recent developments of medical and surgical research (2023), pp. 8 – 15
ISSN: 2791-0814 (online) 2791-0806 (Print)
DOI: 10.30538/psrp-tmcs2023.si-rdmsr002
ISSN: 2791-0814 (online) 2791-0806 (Print)
DOI: 10.30538/psrp-tmcs2023.si-rdmsr002
Role of P53 and KI67 in oral squamous cell carcinoma: An immunohistochemical study
Suvvari Jagadeeswari\(^{1}\), Tutta Kishore Kumar\(^{1}\), Thatipakala Ramya Durga\(^{1}\), Hanumanthu Lakshmi Vasavi\(^{2,*}\) and Vijaya Bharathi\(^{3}\)
\(^{1}\) Department of Pathology, Government Medical College, Balaga Road Srikakulam-532001, Andhra Pradesh, India.
\(^2\) Department of Pathology, Andhra Medical College, Visakhapatnam -530002, India.
\(^3\) Department of Pathology, Government Medical College, Balaga Road Srikakulam-532001, Andhra Pradesh, India.
Correspondence should be addressed to Hanumanthu Lakshmi Vasavi at vasavihanumanthu397@gmail.com
Copyright © 2023 Suvvari Jagadeeswari, Tutta Kishore Kumar, Thatipakala Ramya Durga, Hanumanthu Lakshmi Vasavi and Vijaya Bharathi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: December 25, 2022 – Accepted: February 9, 2023 – Published: February 28, 2023
Abstract
Background: Oral squamous cell carcinoma is a carcinoma with squamous differentiation arising from the mucosal epithelium. The pathogenesis of oral cancers is multifactorial. P53, guardian of the genome regulates cell cycle progression, DNA repair, cellular senescence and apoptosis. Ki-67 is a cell cycle associated nuclear protein used as a proliferation marker to measure the growth fraction of cells in human tumours.
Aims and Objectives: To compare demographic factors like age, sex and predisposing factors. To evaluate grade of differentiation, expression of p53, Ki67 and their comparison in various grades of differentiation in oral squamous cell carcinomas.
Materials and Methods: This study comprises of 35 cases of oral squamous cell carcinoma After processing of representative tissue block, H\&E and IHC stain with Ki-67 and p53 immunomarkers were carried out.
Results: Out of 35 cases of oral squamous cell carcinoma,20(57%) were well differentiated, 14(40%) moderately differentiated, 1(3%) was poorly differentiated. The age range was 26-85 years. Sex ratio was 2:1. The most common risk factor for development of cancer was found to be smoking (57%) followed by betel quid chewing (43%). The most common clinical presentation was non healing ulcer (80%). Sensitivity of Ki67 and P53 is 100% and 63% respectively.
Conclusion: P53 positivity was demonstrated in majority of cases indicating that it is most common genetic mutations in oral cancers. The present study showed an inverse correlation between the degree of tumour differentiation and the rate of cell proliferation obtained by the expression of Ki-67.
Aims and Objectives: To compare demographic factors like age, sex and predisposing factors. To evaluate grade of differentiation, expression of p53, Ki67 and their comparison in various grades of differentiation in oral squamous cell carcinomas.
Materials and Methods: This study comprises of 35 cases of oral squamous cell carcinoma After processing of representative tissue block, H\&E and IHC stain with Ki-67 and p53 immunomarkers were carried out.
Results: Out of 35 cases of oral squamous cell carcinoma,20(57%) were well differentiated, 14(40%) moderately differentiated, 1(3%) was poorly differentiated. The age range was 26-85 years. Sex ratio was 2:1. The most common risk factor for development of cancer was found to be smoking (57%) followed by betel quid chewing (43%). The most common clinical presentation was non healing ulcer (80%). Sensitivity of Ki67 and P53 is 100% and 63% respectively.
Conclusion: P53 positivity was demonstrated in majority of cases indicating that it is most common genetic mutations in oral cancers. The present study showed an inverse correlation between the degree of tumour differentiation and the rate of cell proliferation obtained by the expression of Ki-67.
Keywords:
OSCC-Oral squamous cell carcinoma.