OJC-Vol. 1 (2018), Issue 1, pp. 01–11 |
Open Access Full-Text PDFSajid Mahmood, Muhammad Arshad, Zaheer Ahmed
Abstract:The objective of the present work was to develop and validate of an analytical method for the quantitative determination of Fexo. HCL and Pseudo. HCL in a combine tablet dosage form by \(UV-V\) is spectrophotometry and TLC. The main problem was to separate the two active ingredient from a single bilayered tablet because both the A.P.I’s were soluble in the same solvents. As media selection, distilled water and ethanol \((1:1)\) were used for Pseudo. HCl and methanol for Fexo. HCl, in which both the drugs were soluble and stable for a sufficient time. Both drugs were measured at \(220\)nm and \(247\)nm, where they showed maximum absorbance. Beer Lambert’s law was obeyed at concentration range \(4-14\) ppm and \(5-30\) ppm for Fexo. HCL and Pseudo HCL respectively. Fexo. HCl \((Y=0.0643x+0.9370)\) was measured with correlation coefficient \(r =0.9574\) and Pseudo. HCl \((Y=0.0843x+0.0219)\) with correlation coefficient \(r =0.9992\). The results of analysis have been validated statistically and recovery studies were carried out as \(99.29\%\pm 0.943\) and \(99.29\%\pm 0.941\) which were close to the assay value \(100.1\% \& 100.6 \%\). Precision of the method was measured which showed results for SD \((99.57 \% \;\;\& \;\;99. 51% )\) and \(\%\) RSD \((99.53 \%\;\; \&\;\; 99.54)\). The proposed method may be suitably applied for the analysis of Fexo. HCL and Pseudo.HCL in tablet pharmaceutical formulation for routine analysis.
