Trends in Clinical and Medical Sciences
Special issue: Indian Medical Research Views and Findings on Last 5 years (2023), pp. 229 – 233
ISSN: 2791-0814 (online) 2791-0806 (Print)
DOI: 10.30538/psrp-tmcs2023.si-imrv035
ISSN: 2791-0814 (online) 2791-0806 (Print)
DOI: 10.30538/psrp-tmcs2023.si-imrv035
Bacteriological profile and their antimicrobial susceptibility pattern in the periodontal lesions
Dr. Pallavi Jain\(^{1}\), Dr. Parul Nema\(^{2}\), Dr. Abhishek Kumar Jain\(^{3}\) and Dr. Ramesh Agrawal\(^{4*}\)
\(^{1}\) MDS Periodontology, Junior Resident, Department of Dentistry, RVRS Govt. Medical College, Bhilwara, Rajasthan.
\(^{2}\) Assistant Professor, Department of Pathology, SRVS Medical College, Shivpuri, M.P.
\(^{3}\) Assistant Professor, Department of Microbiology, RVRS Govt. Medical College, Bhilwara, Rajasthan.
\(^{4}\) Assistant Professor, Department of Microbiology, NSC Govt. Medical College, Khandwa, M.P.
Correspondence should be addressed to Dr. Ramesh Agrawal at drrameshagrawal22@gmail.com
Copyright © 2023 Dr. Pallavi Jain, Dr. Parul Nema, Dr. Abhishek Kumar Jain and Dr. Ramesh Agrawal. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: February 13, 2023 – Accepted: May 5, 2023 – Published: May 24, 2023
Abstract
Context: Periodontal disease is a disease, or more likely a number of diseases of the periodontal tissues that results in attachment loss and destruction of alveolar bone. The dental biofilm is responsible for the pathogenesis of the periodontal lesions, resulting both from the direct degradative action and the indirect inflammatory lesions, mediated by microbial cells and their virulence factors.
Aims:The aims of this prospective study were the isolation, identification and characterization of the antibiotic susceptibility profiles of bacterial isolated from patients with periodontal lesions.
Material and Methods: This prospective and non-randomized study was carried out at Department of Periodontology, MPCD & RC, Gwalior and Microbiology, G. R. M. C., Gwalior, Madhya Pradesh for the period of one and half years. The study was conducted for the period of 1 and half year. During the period a total 50 samples were collected following inclusion and exclusion criteria and processed as per Clinical and Laboratory Standards Institute guidelines. The statistical analysis was performed by SPSS software.
Results: A total of 50 study subjects were included, with culture positivity rate of 36% (18). Male to female ratio was 2:1 with mean age of 38.1 years. E. coli, Staphylococcus aureus, Streptococcus mutant, Klebsiella species and Candida species were isolated. Maximum resistant was observed against ceftriaxone (60%), ceftazidime (53%), amoxicillin clavulanate (46%) and low resistance against gentamicin, ciprofloxacin and doxycycline.
Conclusion: The antibiotic susceptibility assay revealed different profiles and significant levels of antimicrobial resistance, reflecting the necessity to perform the microbiological analysis and the antibiotic susceptibility testing in order to select the optimal antimicrobial therapy for the treatment of the periodontal disease.
Aims:The aims of this prospective study were the isolation, identification and characterization of the antibiotic susceptibility profiles of bacterial isolated from patients with periodontal lesions.
Material and Methods: This prospective and non-randomized study was carried out at Department of Periodontology, MPCD & RC, Gwalior and Microbiology, G. R. M. C., Gwalior, Madhya Pradesh for the period of one and half years. The study was conducted for the period of 1 and half year. During the period a total 50 samples were collected following inclusion and exclusion criteria and processed as per Clinical and Laboratory Standards Institute guidelines. The statistical analysis was performed by SPSS software.
Results: A total of 50 study subjects were included, with culture positivity rate of 36% (18). Male to female ratio was 2:1 with mean age of 38.1 years. E. coli, Staphylococcus aureus, Streptococcus mutant, Klebsiella species and Candida species were isolated. Maximum resistant was observed against ceftriaxone (60%), ceftazidime (53%), amoxicillin clavulanate (46%) and low resistance against gentamicin, ciprofloxacin and doxycycline.
Conclusion: The antibiotic susceptibility assay revealed different profiles and significant levels of antimicrobial resistance, reflecting the necessity to perform the microbiological analysis and the antibiotic susceptibility testing in order to select the optimal antimicrobial therapy for the treatment of the periodontal disease.
Keywords:
Periodontitis; Biofilm; Periodontal pathogens.